Coding

Part:BBa_K1344010:Design

Designed by: UI Indonesia 2014   Group: iGEM14_UI-Indonesia   (2014-10-06)


Reversed histidine kinase quorum sensing system from vibrio cholerae


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 868
    Illegal XbaI site found at 2038
    Illegal SpeI site found at 28
    Illegal SpeI site found at 2026
    Illegal PstI site found at 1431
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 868
    Illegal SpeI site found at 28
    Illegal SpeI site found at 2026
    Illegal PstI site found at 1431
    Illegal NotI site found at 2012
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 868
    Illegal BamHI site found at 16
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 868
    Illegal XbaI site found at 2038
    Illegal SpeI site found at 28
    Illegal SpeI site found at 2026
    Illegal PstI site found at 1431
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 868
    Illegal XbaI site found at 2038
    Illegal SpeI site found at 28
    Illegal SpeI site found at 2026
    Illegal PstI site found at 1431
    Illegal AgeI site found at 142
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1552


Design Notes

This is quorum sensing parts


Source

From Vibrio cholerae genome

References